Analysis Note

ControlHuman IL-6 recombinant protein

Application

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Anti-Interleukin-6 (IL-6) Antibody, clone 1G3 is an antibody against Interleukin-6 (IL-6) for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that seems to be involved in the late stages of CTL generation. Other biological functions also include amplification and the inflammatory response. Combination therapies including IL-6 have been shown to have positive effects on specific malignant tumor types. The accumulation of IL-6 in the CNS is usually detrimental and can contribute to pathology of CNS diseases. IL-6 signaling is negatively regulated by TGF-beta, which down regulates STAT1, STAT3 and ICAM-1 expression. IL-6 expression has been linked to inflammatory bowel disease (IBD) and is also the primary clinical target for IBD. Clinical use of an antibody for the IL-6 receptor (IL-6R) has been promising in treating rheumatoid arthritis, systemic juvenile idiopathic arthritis and Castleman's disease.

Immunogen

Human IL-6 fused to the soluble domain of the IL-6R.

Linkage

Replaces: 04-1095

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot with human IL-6 recombinant protein.Western Blot Analysis: 0.5 g/mL of this antibody detected 10 g of human IL-6 recombinant protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~21 kDa observed